Sources of contaminant uptake in fishes are primarily through diet and skin absorption. Evidence from various marine organisms, mainly elasmobranchs, suggest contaminants are transferred from the mother to developing eggs/fetuses, a mechanism termed maternal offloading, through lipid transfer, the main source of nutrition to growing eggs. New evidence suggests a pathway for males to offload contaminants through sperm, via lipids they contain, which fertilizes the egg. Chemicals of Emerging and Known Concern (CECs and CKCs, resp.), including PAHs, PCBs, and organochlorine pesticides (OCPs) are known to be lipophilic—preferentially binding to lipids. This study examines the potential for parental offloading of CECs and CKCs in Spotted Seatrout (SST), Cynoscion nebulosis, occupying a highly urbanized estuary, Tampa Bay, along the Gulf coast of Florida. Fish were collected via rod and reel fishing throughout one year with a stratified sampling design in the Bay, with ten fish collected across six regions every quarter. A total of 249 SST were collected, males and females, ranging in size from 23.0-56.3 cm and ages 0-4 years old. Fish were measured for total length (cm) and weighed (g) whole body, gonad, and liver. Accelerated Solvent Extraction (ASE) analyses measured lipid content of livers and gonads, which vary seasonally, peaking prior to spawning (Apr-Sept) and declining thereafter as the fish mobilizes energy resources for the developing eggs. Contaminants are thought to be transferred to eggs during this process. Gas chromatography with tandem mass spectrometry (GC/MS/MS) is used to quantify the presence and concentrations of CECs and CKCs. We expect high concentrations of pollutants in SST tissues and will report on the seasonal and spatial variation in these concentrations in relation to hypothesized pollutant offloading. SST are important to recreational and subsistence fisheries, thus the health of both the fish and fish-consuming-public need to be well understood.