Seagrass meadows play a fundamental role in mitigating climate change by sequestering carbon within their sediments through photosynthetic processes, limiting carbon exchange with the atmosphere. Seagrasses are typically net sources of alkalinity and can therefore buffer ocean acidification, reducing detrimental effects on calcifying marine organisms. Despite this high capacity for carbon storage and alkalinity generation, there remains a research gap regarding how subtropical seagrass meadows perform these ecosystem functions in the Gulf of America. Incorporating different components of the carbon cycle including dissolved inorganic carbon (DIC), total alkalinity (TA), dissolved organic carbon (DOC), pCO2, and nutrients can provide a more comprehensive understanding of the total carbon budget for the Mississippi Sound. We hypothesized that seagrass beds in Mississippi would exhibit positive TA/DIC ratios, with values varying depending on the prevalent species and seagrass distribution at each site. To test this, we analyzed samples from over 80 locations across four barrier islands: Cat Island, Ship Island, Horn Island, and Petit Bois Island. Preliminary results from DIC and TA analyses suggest that the sampled seagrass meadows were net sources of TA, with TA/DIC ratios exceeding 1 across all four islands. Measurements of DOC, pCO2, CH4, and nutrients, are also analyzed and compared with environmental parameters such as salinity, temperature, dissolved oxygen, and pH, as well as physical seagrass meadow conditions, percent cover, species composition, and so on. This study contributes to the limited body of research on subtropical seagrass blue carbon dynamics and provides the first known dataset for this region. The findings highlight the importance of carbon sequestration, transport, and transformation as an ecosystem service of seagrass meadows, offering valuable insights to inform potential restoration and conservation efforts in the Mississippi Sound.