The Mississippi Sound and Bight (MSB) is a rich and complex area of the northern Gulf impacted by multiple stressors. Much of the western portion of the MSB has oil extraction and production infrastructure, leading to numerous oil spills of various size occurring in recent decades , which can act as both acute and chronic stressors in the environment . The Natural Resource Damage Assessment (NRDA) process quantifies injuries to natural resources from spills and scales these injuries to projects that restore for the losses from the spill. Data collection efforts associated with a recent spill in the Pass a Loutre Wildlife Management Area highlight how silicone band passive sampling devices can be used to quantify contaminant exposures and associated injuries to water column resources resulting from oil releases. The passive samplers characterize time-integrated exposure to bioavailable PAHs (polycyclic aromatic hydrocarbons) in water that organisms experience as a result of an oil spill, versus traditional grab water sampling, which only provides a snapshot of total contaminant concentrations. Following the spill, the uptake of PAHs in passive samplers correlated with observed shoreline and sediment oiling, in addition to site hydrology. Data collected at adjacent reference sites during the incident highlight the direct increases in water column concentrations of PAHs resulting from acute releases of oil, and comparison with data from sites further away in the MSB help put into context the low-level chronic contaminants vs the acutely elevated PAHs during a spill. Water column resources exposed to the levels of PAHs measured during the spill are known to cause effects that range from direct mortality (e.g. early life stages) to sublethal effects.