Bats provide invaluable ecological services but are facing population threats throughout the southeast region. As threats such as white-nose syndrome and loss of roost habitat increase, the importance of identifying and protecting habitats utilized by bats is increasing. This project focused primarily on identifying presence and habitat-use by monitoring activity of the endangered northern long-eared bat (Myotis septentrionalis) and proposed endangered tricolored bat (Perimyotis subflavus) on the Mississippi coast. Both these species have been extremely impacted by white-nose syndrome because of their hibernation behaviors during winter months. They are susceptible to loss and disturbance of maternity roosting habitat by urban development, but data on warm month habitat-use in coastal Mississippi is lacking. Identifying species presence and monitoring activity within coastal habitats is vital for bat conservation efforts. This project acoustically monitored and surveyed across the Mississippi coast to identify species presence and activity in coastal forest areas. This work is the first long-term tricolored and northern long-eared bat focused monitoring project on the Mississippi coast. This project will improve understanding of the distribution, activity, and habitat preference of the tricolored and northern long-eared bat in coastal Mississippi. The findings from this proposed study would benefit nation-wide efforts in protecting tricolored and northern long-eared bat habitat, especially for identifying summer roost habitats and improved understanding of ecology of these species along the northern US Gulf coast.