Persistent organic pollutants (POPs) encompass a wide range of legacy and in-use contaminants such as pesticides, PCBs, pharmaceuticals, flame retardants, and polycyclic aromatic hydrocarbons (PAHs) with the potential to cause long term ecological harm. Tampa Bay with its unique interplay of hydrological (e.g., four major estuarine tributaries) and social (high tourist/economic impact, military presence, five wastewater treatment plants, large urban population, etc.) parameters make it a likely source for POPs within the Bay and adjacent coastal areas. POPs have an affinity for marine sediments due to their interactions with organic matter, but sediment accumulation is also influenced by hydrological parameters and sedimentary characteristics (e.g., surface charge, pH, granular composition). For this study, 13 sediment cores and surface sediments from 17 sites were collected around Tampa Bay between May of 2024 and October of 2025. To characterize Tampa Bay study sites, anthropogenic and natural environmental factors were mapped using Geographic Information Systems (GIS). The bay was divided into six sub-regions: Old Tampa Bay, Hillsborough Bay, mid-Bay east and west, and lower bay east and west. Datasets were obtained from the USGS National Water Dashboard and Tampa Bay Water Atlas covering from January 2020 to October 2025, corresponding to the surface sediment layer deposited over the past five years. Study sites were superimposed with the anthropogenic and environmental factors to generate maps summarizing the multiple potential sources of various POPs. We will discuss how each sub-region within Tampa Bay presents a unique environmental signature, driven by varying combinations of natural processes and human activities that shape spatial patterns of POP contamination and accumulation.