​Crayfish (also called crawdads and mudbugs) are decapod crustaceans of the infraorder Astacidea and are separated into two superfamilies depending on hemisphere: Astacoidea for Northern and Parastacoidea for Southern. North America is home to most ofastacoidean diversity, with the Southeastern United States being particularly diverse. Among many crayfish rich states, Mississippi houses an impressive roster of crayfish species with more than ~80 known species, the fourtj most of any US state.. Despite this, its crayfish populations are understudied, with uncertainty surrounding their taxonomy, species boundaries, and more. This study seeks to examine population biology and crypic speciation in the genus Procambarus, and with particular focus on subspecies Pennides, such as the species: Procambarus vioscai, P. clemmeri, and P. penni, to name a few. These crayfish have significant population overlap despite disjunct distributions across the state of Mississippiand neighboring states and morphologically, putative species are nearly identical.   While field surveys, genetic analyses, and morphometric analyses are underway, here we examine existing knowledge of the groups biology, morphology, and ecology to highlight ongoing need for further work.